Protein Synthesis in Single Cell Suspensions from Rat Liver
نویسندگان
چکیده
منابع مشابه
[Protein synthesis in single-cell suspensions from rat liver].
Suspensions of single cells were prepared from normal adult rat liver by perfusion of liver with and shaking in buffered solutions of 0.1% hyaluronidase and 0.05 % collagenase. L-[lJ4C]Leucine and L-[lJ4C]methionine incorporated into protein could be measured by precipitating the cells on filter paper disks without prior homogenization. No incorporation of amino acids into protein was observed ...
متن کاملProtein and ribonucleic acid metabolism in single-cell suspensions from Morris hepatoma 5123tc and from normal rat liver.
kinetics similar to those found for liver in the intact animal. This is in contrast to the lack of secretion of serum proteins by the hepatoma observed in vivo. The transfer was not inhibited when protein synthesis was interrupted by puromycin or cycloheximide or when the cells were incubated under nitrogen. U ridine, uracil, and orotic acid were efficiently incorpo rated into the RNA of hepato...
متن کاملPreparation of single-cell suspensions from normal liver, regenerating liver, and Morris hepatomas 9121 and 5123tc.
Suspensions of single cells were prepared from normal adult rat liver either by mincing the tissue and incubating it in various concentrations of sodium tetraphenylborate, ethylenediaminetetraacetate, and 0.1% hyaluronidase plus 0.05% collagenase or by continuous recirculating perfusion of the liver with 0.1% hyaluronidase plus 0.05% collagenase. Only the latter method produced cells in good yi...
متن کاملProtein Synthesis in the Perfused Rat Liver
1. When the rat livers are perfused under the conditions of these experiments with rat blood diluted with saline, the livers remain capable of removing colloidal chromic phosphate normally for 4 hours or more; that is, the reticuloendothelial system continues to function normally. 2. Good rates of bile flow continue, generally for 4 hours. 3. The livers incorporate radioactivity from the amino ...
متن کاملBiotin and protein synthesis in rat liver.
Biotin administration to biotin-deficient rats enhanced protein synthesis. Time sequence studies indicated that RNA synthesis was stimulated as early as 1 hour after biotin administration to the deficient rat. The synthesis of RNA of liver was stimulated by biotin administration. Nuclear RNA isolated from biotin-injected rats was more active in a protein-synthesizing system in vitro than a simi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44802-3